https://technology.pagepress.org/index.php/phycol/issue/feed Phycology International 2019-03-19T11:20:23+01:00 Francesca Baccino francesca.baccino@pagepress.org Open Journal Systems <p><strong>Phycology International</strong> is a new, online electronic journal covering all domains of phycology, including the contributions of phycological research to current and emerging issues in environmental, health and industrial sectors. Works on both eukaryotic algae and cyanobacteria fall within the scope of the journal. The subject areas covered include, among others: Applied phycology and biotechnology, Morphology and cell biology, Macro- and microalgal ecology, Macro- and microalgal systematics and evolution, Molecular biology and genomics, Physiology and biochemistry, Toxicology, Health and risk management, Algal systems for waste water treatment, Biogas production, Algae in recovery of metals, Role of algae in medicine. The journal publishes original articles, reviews, and technical notes.</p> <p>This journal is fully Open Access, it has no submission fee and no article processing charge.&nbsp;All papers are immediately published as soon as they have been accepted, by adding them to the "current" volume's Table of Contents.<br><br>Looking for rapid publication? Fair peer-review? Immediate indexing? <a href="/technology/index.php/phycol/about/submissions"><strong>Submit your paper to our journal!</strong></a></p> https://technology.pagepress.org/index.php/phycol/article/view/62 Comparative characteristics of cytosolic and chloroplastidial D/L- myo-Inositol-1-phosphate phosphatase partially purified from Enteromorpha intestinalis (L.) Nees (a marine macro alga) grown under high salinity niche 2019-03-19T11:20:22+01:00 Sautrik Basu basusautrik2018@gmail.com Anusuya Basak basusautrik2018@gmail.com Dibyendu Sekhar Mahanty basusautrik2018@gmail.com Sayani Bhattacharjee basusautrik2018@gmail.com Jukta Adhikari basusautrik2018@gmail.com <p>The present investigation describes a protocol for partial purification and basic characterization of one of the key enzymes of <em>myo</em>-inositol biosynthesis, D/L- <em>myo</em>- Inositol-1-phosphate phosphatase (MIPP), from a marine macro alga (<em>Enteromorpha intestinalis</em>) growing under varying salinity conditions of Chilika lagoon (Odisha, India). Cytosolic and chloroplastidial forms of MIPP were partially purified to about 55- and 16- folds respectively following lowspeed centrifugation, high-speed centrifugation, 0-80% ammonium sulfate precipitation, successive chromatography through CM-cellulose, Sephadex G-200 and UltrogelAcA 34 columns. The apparent molecular weights of the native cytosolic and plastidial forms of MIPP were determined to be 146 and 148 kDa respectively. Cytosolic and plastidial MIPP were remarkably active within the temperature range of 20-40°C and function within a narrow pH range (7.0-7.5). Using nonlinear regression kinetics, the Km value for D-MIP of the cytosolic MIPP and its plastidial iso-form were 0.07277 mM and 0.07332 mM respectively. Different monovalent as well as divalent cations exhibited variable effects on enzyme activity of either preparation. The activity of MIPP was remarkably found to increase proportionately with the salinity of Chilika lagoon.</p> 2019-03-19T11:20:14+01:00 ##submission.copyrightStatement##