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Investigating the microenvironmental effects of scaffold chemistry and topology in human mesenchymal stromal cell/polymeric hollow microfiber constructs

Claudio Ricci, Luisa Trombi, Ilaria Soriga, Fabio Piredda, Mario Milazzo, Cesare Stefanini, Luca Bruschini, Giuseppe Perale, Gianni Pertici, Serena Danti
  • Claudio Ricci
    OtoLab, Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy
  • Luisa Trombi
    OtoLab, Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy
  • Ilaria Soriga
    Department of Chemistry, Materials and Chemical Engineering, Politecnico di Milano, Milan, Italy
  • Fabio Piredda
    Department of Chemistry, Materials and Chemical Engineering, Politecnico di Milano, Milan, Italy
  • Mario Milazzo
    Creative Engineering Design Area, Biorobotics Institute, Scuola Superiore Sant’Anna, Pontedera (PI), Italy
  • Cesare Stefanini
    Creative Engineering Design Area, Biorobotics Institute, Scuola Superiore Sant’Anna, Pontedera (PI), Italy; Department of Biomedical Engineering, Khalifa University, Abu Dhabi, United Arab Emirates
  • Luca Bruschini
    OtoLab, Azienda Ospedaliero-Universitaria Pisana, Pisa; Department of Surgical, Medical, Molecular Pathology and Emergency Medicine, University of Pisa, Pisa, Italy
  • Giuseppe Perale
    Department of Innovative Technologies, University of Applied Sciences and Arts of Southern Switzerland, Manno, Switzerland
  • Gianni Pertici
    Department of Innovative Technologies, University of Applied Sciences and Arts of Southern Switzerland, Manno, Switzerland
  • Serena Danti http://orcid.org/0000-0002-8155-8537
    OtoLab, Azienda Ospedaliero-Universitaria Pisana, Pisa; Creative Engineering Design Area, Biorobotics Institute, Scuola Superiore Sant’Anna, Pontedera (PI); Department of Civil and Industrial Engineering, University of Pisa, Pisa, Italy | s.danti@med.unipi.it

Abstract

Tissue engineering scaffolds have shown an intrinsic ability to provide cellular stimulation, thus behaving as physically active microenvironments. This study reports on the interaction between human mesenchymal stromal cells (hMSCs) and dry-wet spun polymer microfiber meshes. The following scaffolding parameters were tested: i) polymer type: poly-L-lactide (PLLA) vs poly-ε-caprolactone (PCL); ii) non-solvent type: ethanol (Et-OH) vs isopropanol/ gelatin; iii) scaffold layout: patterned vs random microfiber fabrics. After two culture weeks, the effects on metabolic activity, scaffold colonization and function of undifferentiated hMSCs were assayed. In our study, the polymer type affected the hMSC metabolic activity timeline, and the metabolic picks occurred earlier in PLLA (day 6) than in PCL (day 9) scaffolds. Instead, PLLA vs PCL had no endpoint effect on alkaline phosphatase (ALP) activity expression. On average, the hMSCs grown on all the random microfiber fabrics showed an ALP activity statistically superior to that detected on patterned microfiber fabrics, with the highest in Et-OH random subtypes. Such findings are suggestive of enhanced osteogenic potential. The understanding of scaffold-driven stimulation could enable environmental hMSC commitment, paving the way for new regenerative strategies.

Keywords

Poly(L-lactic) acid; Poly-caprolactone; Dry-wet spinning; Viability; Tissue engineering

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Submitted: 2015-12-07 16:14:48
Published: 2016-05-30 00:00:00
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Copyright (c) 2016 Claudio Ricci, Luisa Trombi, Ilaria Soriga, Fabio Piredda, Mario Milazzo, Cesare Stefanini, Luca Bruschini, Giuseppe Perale, Gianni Pertici, Serena Danti

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